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Cryo Electron Microscopy in Structural Biology - Einzelansicht

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Veranstaltungsart Vorlesung/Seminar Veranstaltungsnummer
SWS 4 Semester WiSe 2021/22
Einrichtung Institut für Biochemie und Biologie   Sprache englisch
Belegungsfrist 01.10.2021 - 10.11.2021   
Gruppe 1:
     Zur Zeit keine Belegung möglich
    Tag Zeit Rhythmus Dauer Raum Lehrperson fällt aus am Max. Teilnehmer/-innen
Einzeltermine anzeigen
Vorlesung Di 08:15 bis 09:45 wöchentlich 26.10.2021 bis 15.02.2022  2.25.B0.01 Prof. Dr. Wendler 21.12.2021: Akademische Weihnachtsferien
28.12.2021: Akademische Weihnachtsferien
Einzeltermine anzeigen
Seminar Di 10:15 bis 11:45 wöchentlich 26.10.2021 bis 15.02.2022  2.25.B0.01 Dr. rer. nat. Petrovic ,
Dr. rer. nat. Radon ,
Prof. Dr. Wendler
21.12.2021: Akademische Weihnachtsferien
28.12.2021: Akademische Weihnachtsferien
Kommentar

Content:
The course covers theory and advanced image analysis techniques in transmission electron microscopy (TEM). In the lecture the following topics are explained:
- buildup of a TEM, electron guns, holders and detectors
- theory of diffraction, image formation, how electrons interact with material; contrast transfer function
- theory behind high resolution cryo TEM
- sample preparation and image acquisition in (single particle) TEM
- 2D and 3D image analysis
- point group symmetries
- refinement and validation of 3D reconstructions
- visualisation and interpretation of TEM results

During the seminar the students will analyse current TEM results from subject-specific English literature, summarize the key aspects of the work and discuss the research outcome critically.

The practical course will cover hands-on practical work on the transmission electron microscope as well as single-particle image processing of a high-resolution cryo EM data set. The TEM work will include
- negative staining of a protein complex
- loading of the grid into the NS holder
- setting up a negative stain data collection in the data acquisition software
The image processing will be undertaken on the teaching server of the IBB using the computer pools in building 25 (Campus Golm). Students will learn how to work with the linux based server system and the EM processing software Relion. Students will learn how to assess the quality of the data and how to get from individual images to a high-resolution 3D model of the protein complex.

Learning outcomes:
1. Subject-specific competences:
At the end of the course the students will have learned and understood the underlying theory in transmission electron microscopy and single particle image analysis. They will be able to identify suitable TEM applications to a biological question and will have gained insight into the current state of the art in cryo electron microscopy. They will also have analysed the structure and function of diverse biological complexes.
2. Methods-specific competences:
The students learn to interpret, analyse and present results derived from subject-specific, English literature. They will also learn how to prepare a TEM sample, how to operate a TEM, how to analyse TEM images and how to interpret TEM data.
3. Action competence:
The students present and defend scientific work in a public seminar using suitable presentation media. The students learn to work in a team, give constructive feedback and assess each other in a public seminar. When participating in the practical course students will learn to collect and assess scientific data. Furthermore they will summarize experimental data in a detailed protocol and evaluate their results.


Strukturbaum
Die Veranstaltung wurde 8 mal im Vorlesungsverzeichnis WiSe 2021/22 gefunden:
Vorlesungsverzeichnis
Mathematisch-Naturwissenschaftliche Fakultät
Institut für Biochemie und Biologie
Master of Science
Biochemistry and Molecular Biology (Prüfungsversion ab WiSe 2016/17)
Wahlpflichtmodule
BIO-B-WM1 - Biochemistry A  - - - 1
BIO-B-WM3 - Protein Science A  - - - 2
BIO-B-WM4 - Genome Research and Systems Biology A  - - - 3
BIO-B-WM7 - Biochemistry B  - - - 4
BIO-B-WM9 - Protein Science B  - - - 5
BIO-B-WM10 - Genome Research and Systems Biology B  - - - 6
Richtungsmodule
BIO-B-RM22 - Current Research in Biochemistry and Molecular Biology in Local Research Institutes and Biotechnology Companies  - - - 7
Ecology, Evolution and Conservation (Prüfungsversion ab WiSe 2019/20)
Elective modules B
BIO-B-WM10 - Genome Research and Systems Biology B  - - - 8